Actin-Like 7b (Actl7b) antibody

Antigen: Actin-Like 7b (Actl7b)

Synonyms: Tact1, MGC151192, MGC151194, ACTL7B

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238621

Additional Information

Alternative name: Actin-like 7B

Gene ID: 10880

Immunogen: C-QLKMKPRKVHKIK

Format: Affinity-purified

Description: All names and symbols: actin-like 7B, ACTL7B, RP11-3J11.2, actin-like 7-beta. Official Symbol: ACTL7B. RefSeq number(s): NP_006677.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 48kDa band observed in human testis lysates (calculated MW of 45.2kDa according to NP_006677.1). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN238621 (0.03µg/ml) staining of human testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Chadwick, Mull, Helbling et al.: "Cloning, mapping, and expression of two novel actin genes, actin-like-7A (ACTL7A) and actin-like-7B (ACTL7B), from the familial dysautonomia candidate region on 9q31." in: Genomics, Vol. 58, Issue 3, pp. 302-9, 1999 (PubMed).