Neuroligin 3 (NLGN3) antibody

Antigen: Neuroligin 3 (NLGN3)

Synonyms: HNL3, ASPGX1, AUTSX1, KIAA1480, NL3, A230085M13Rik, Nlgn3, GB18290, nlgn3, MGC84475, NLGN3, MGC143069, LOC574216, CCE-L1, NV16744, Nv1604741

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238626

Additional Information

Alternative name: Neuroligin 3

Gene ID: 54413

Immunogen: C-RKDKRRQEPLRQPS

Description: All names and symbols: neuroligin 3, KIAA1480, ASPGX1, AUTSX1, HNL3, NLGN3. Official Symbol: NLGN3. RefSeq number(s): NP_061850.2

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx. 85-90kDa band observed in Human Brain (Amygdala) lysates (calculated MW of 91.6kDa according to NP_061850.2). In transfected HEK293 transiently expressing Neuregulin3 a band of approx. 98kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.05-0.2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Neurology, Glia marker

Restrictions: For Research Use only

Images

ABIN238626 (0.1µg/ml) staining of Human Brain (Amygdala) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Tabuchi, Blundell, Etherton et al.: "A neuroligin-3 mutation implicated in autism increases inhibitory synaptic transmission in mice." in: Science (New York, N.Y.), Vol. 318, Issue 5847, pp. 71-6, 2007 (PubMed).