PiggyBac Transposable Element Derived 2 (PGBD2) antibody

Antigen: PiggyBac Transposable Element Derived 2 (PGBD2)

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238633

Additional Information

Alternative name: PGBD2

Gene ID: 267002

Immunogen: C-ESNADTTSQGRRSRR

Format: Affinity-purified

Description: All names and symbols: PGBD2, piggyBac transposable element derived 2, hypothetical protein LOC267002. Official Symbol: PGBD2 . RefSeq number(s): NP_001017434.1, NP_733843.1

Specificity: This antibody is expected to recognise both reported isoforms (NP_001017434.1, NP_733843.1) Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx 30kDa band in Human Brain (Cerebral Cortex) lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 39.6kDa according to NP_001017434.1 and of 68.0kDa according to NP_733843.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only

Publications

Publications: Wu, Meir, Coates et al.: "piggyBac is a flexible and highly active transposon as compared to sleeping beauty, Tol2, and Mos1 in mammalian cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 41, pp. 15008-13, 2006 (PubMed).