Apolipoprotein B MRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A) antibody

Antigen: Apolipoprotein B MRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A)

Synonyms: APOBEC3A, APOBEC3Z1, ARP3, PHRBN, bK150C2.1

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238645

Additional Information

Alternative name: Phorbolin 1 / APOBEC3A

Gene ID: 200315

Immunogen: C-TSNFNNGIGRHKTY

Format: Affinity-purified

Description: All names and symbols: APOBEC3A, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A, ARP3, PHRBN, bK150C2.1, phorbolin 1. Official Symbol: APOBEC3A . RefSeq number(s): NP_663745.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Preliminary experiments gave an approx 28kDa band in Human Spleen, Tonsil and Peripheral Blood Mononucleocyte lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 23.0kDa according to NP_663745.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Goila-Gaur, Khan, Miyagi et al.: "Targeting APOBEC3A to the viral nucleoprotein complex confers antiviral activity." in: Retrovirology, Vol. 4, pp. 61, 2007 (PubMed).