Retinal Pigment Epithelium 65 (RPE65) antibody

Antigen: Retinal Pigment Epithelium 65 (RPE65)

Synonyms: LCA2, RP20, rd12, mRPE65, sRPE65, 65kDa, Mord1, A930029L06Rik, DRPE65, RPE65, anon-WO0148162.1, anon-WO0148163.1, beta-diox, DmelCG9347, CG9347, MGC52887, MGC154296, MGC85437, rpe65

Clonality: Polyclonal

Host: Goat

Reactivity: Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238680

Additional Information

Alternative name: RPE65

Gene ID: 6121

Immunogen: C-EVKKNARKAPQPE

Format: Affinity-purified

Description: All names and symbols: mRPE65, sRPE65, retinal pigment epithelium-specific protein 65kDa, LCA2, RP20, rd12, RBP-binding membrane protein, p63, retinal pigment epithelium-specific 61 kDa protein, retinal pigment epithelium-specific protein (65kD), retinitis pigmentosa 20. Official Symbol: RPE65. RefSeq number(s): NP_000320.1

Specificity: Human, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 60kDa band observed in Rat Retina lysates (calculated MW of 60.9kDa according to Human NP_000320.1 and 61.0kDa according to Rat NP_446014.1). Recommended concentration: 0.5-1.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Metabolism, Neurology

Restrictions: For Research Use only

Images

ABIN238680 (0.5µg/ml) staining of Rat Retina lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Rolling, Le Meur, Stieger et al.: "Gene therapeutic prospects in early onset of severe retinal dystrophy: restoration of vision in RPE65 Briard dogs using an AAV serotype 4 vector that specifically targets the retinal pigmented epithelium." in: Bulletin et m, Vol. 161, Issue 10-12, pp. 497-508; discussion 508-9, 2007 (PubMed).