Neuropeptide FF Receptor 1 (NPFFR1) antibody

Antigen: Neuropeptide FF Receptor 1 (NPFFR1)

Synonyms: NPFF1, GPR147, NPFF1R1, OT7T022, FLJ10751, Gm236, Gpr147, RFRPR, MGC83178, NPFFR1, LOC100230250

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238681

Additional Information

Alternative name: NPFFR1

Gene ID: 64106

Immunogen: C-RPSGSHKEAYSERP

Format: Affinity-purified

Description: All names and symbols: NPFFR1, neuropeptide FF receptor 1 , FLJ10751, GPR147, NPFF1, NPFF1R1, OT7T022, G protein-coupled receptor 147, neuropeptide FF 1. Official Symbol: NPFFR1. RefSeq number(s): NP_071429.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 48kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 47.8kDa according to NP_071429.1). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN238681 (0.03µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Gouarderes, Mazarguil, Mollereau et al.: "Functional differences between NPFF1 and NPFF2 receptor coupling: high intrinsic activities of RFamide-related peptides on stimulation of [35S]GTPgammaS binding." in: Neuropharmacology, Vol. 52, Issue 2, pp. 376-86, 2007 (PubMed).