Xylose antibody

Antigen: Xylose

Clonality: Polyclonal

Host: Rabbit

Reactivity: Higher Plants

Application: Western Blotting (WB)

Catalog no.: ABIN249329

Additional Information

Immunogen: xylose residues bound to the N-glycan in beta 1,2

Format: Lyophilized

Description: This antibody specifically cross-reacts against xylose residues bound to theprotein N-glycans in beta1,2. This residue is characterisitc of the plant proteinN-glycans and is absent in protein N-glycans from animals. This residue is addedin the Golgi apparatus.

Specificity: Predicted reactivity: higher plants.

Application Details

Application Notes: Recommended Dilution 2 ug/10 ml incubation buffer with standard ECL (WB). Additional Information: beta (1,2) xylose is present exclusively in plant N-glycans so antibodies againstthis sugar moiety should not cross-react with any mammal glycoprotein.

Buffer: Affinity purified serum in PBS pH 7.4

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

From left to right: total cell extract from Arabidopsis thaliana wild type and cell extracts from different mutants defective in the complex N-glycan maturation pathway (data not published yet)