14.3.3-Like Protein C (14.3.3C) antibody

Antigen: 14.3.3-Like Protein C (14.3.3C)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Barley (Hordeum vulgare)

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN249336

Additional Information

Immunogen: synthetic peptide specific to the barley C isoform, conjugated to KLH

Format: Lyophilized

Description: 14.3.3’s are 30KDa proteins involved in protein interactions with target proteinscontaining phosphorylated target sites. Functions of 14.3.3’s include acting asadaptors or scaffolds, stimulating protein-protein interaction, altering target proteinactivity, causing conformational changes of target proteins, regulating subcellularlocalisation and also facilitating transport (for example nuclear import/export andtransport in the endomembrane system). A variety of apparently unrelatedbiological activities, including a role in development and signal transduction havebeen ascribed to the 14.3.3 family. To date, five barley 14.3.3 homologues havebeen identified and characterised and named 14.3.3A through E.

Application Details

Application Notes: Recommended Dilution 1: 2000 with standard ECL (WB), 1: 15 000 (ELISA).

Purity: Serum

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

SDS-PAGE gel and duplicate western blot of 1 Hv14.3.3a 2 Hv14.3.3b and 3 Hv14.3.3c, showing anti Hv14.3.3c antibody recognition only Hv14.3.3c protein band.

Publications

Publications: Pertl, Pöckl, Blaschke et al.: "Osmoregulation in Lilium pollen grains occurs via modulation of the plasma membrane H+ ATPase activity by 14-3-3 proteins." in: Plant physiology, Vol. 154, Issue 4, pp. 1921-8, 2010 (PubMed).