XTH-Xet antibody

Catalog No. ABIN249338

There is 1 publication for this product available. The Rabbit Polyclonal anti-XTH-Xet antibody is suitable to detect XTH-Xet in samples from Barley and Oryza sativa. It has been validated for ELISA and WB.

Quick Overview for XTH-Xet antibody (ABIN249338)

Target: XTH-Xet

Reactivity: Barley, Oryza sativa

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB)

Product Details

Purpose: Anti-XTH-Xet | XET5 Xyloglucan xyloglucosyl transferase

Cross-Reactivity (Details): Hordeum vulgare, Oryza sativa
Not reactive in: Poplar sp., Zea mays

Purification: Serum

Immunogen: Two synthetic peptides from highly conserved region of Horderum vulgare XTH-Xet

Application Details

Application Notes: 1 : 5000 (ELISA), 1 : 500 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Hrmova, Farkas, Lahnstein, Fincher: "A Barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-beta-D-glucans." in: The Journal of biological chemistry, Vol. 282, Issue 17, pp. 12951-62, (2007) (PubMed).

Target Details

Target: XTH-Xet

Background: Molecular interactions between wall polysaccharides, which include cellulose and a range of non-cellulosic polysaccharides such as xyloglucans and (1,3,1,4)-?-d-glucans, are fundamental to cell wall properties. These interactions have been assumed to be non-covalent in nature in most cases. A highly purified barley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), a member of the GH16 group of glycoside hydrolases, catalyses the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates, and between xyloglucans and (1,3,1,4)-?-d-glucans. It is possible that XETs could link different polysaccharides in vivo, and hence influence cell wall strength, flexibility and porosity.

Molecular Weight: 31.5 | 33 kDa