Glutamine Oxoglutarate Aminotransferase (GOGAT) antibody

Antigen: Glutamine Oxoglutarate Aminotransferase (GOGAT)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Miscanthus giganteus, Phaseolus vulgaris, Cordgrass (Spartina), Maize/Corn (Zea mays)

Application: Western Blotting (WB)

Catalog no.: ABIN249351

Additional Information

Immunogen: KLH-conjugated synthetic peptide well conserved in known GOGAT sequencesfrom different species including Arabidopsis thaliana Fd-GOGAT 1 Q9ZNZ7-2 andFd-GOGAT 2 Q9T0P4

Format: Lyophilized

Description: Glutamine oxoglutarate aminotransferase (abbreviated as GOGAT) is an enzymeinvolved in synthesis of glutamate from glutamine and alpha-ketoglutarate.GOGAT has two forms in plants: ferredoxin-dependent GOGAT (Fd-GOGAT) andNADH-dependent GOGAT (NADH-GOGAT). 95% of GOGAT found in plants isthe Fd-GOGAT type. Fd-GOGAT is encoded by two genes, glu1 and glu2 foundon chromosomes 5 and 2 respectively (in Arabidopsis). Fd-GOGAT (both forms)is highly conserved among plants, red algae, and cyanobacteria.

Specificity: Predicted reactivity: Medicago trucutula, Ostreococcus lucimarinus, Leptolyngbya boryana, Porphyrapurpurea, Gracilaria tenustipitata, cyanobacteria.

Application Details

Application Notes: Recommended Dilution 1 : 1000 with standard ECL (WB) MW 177 | 170-180 kDa depending upon the species.

Purity: Serum

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

From left to right: Arabidopsis thaliana (1), Phaseaolus vulgaris (2), Zea mays (3), Spartina alterniflora (4), Spartina patens (6). (20 ug of total cellular protein was loaded per lane) A 40 kDa band present in A. thaliana sample is not competed away during antibody neutralization assay. In this assay free peptide used for antibody production is incubated together with anti-GOGAT antibodies.