Clpb Heat Shock Protein (Hsp101) (N-Term) antibody

Antigen: Clpb Heat Shock Protein (Hsp101)

Epitope: N-Term

Clonality: Polyclonal

Host: Chicken

Reactivity: Arabidopsis thaliana

Application: Western Blotting (WB)

Catalog no.: ABIN249369

Additional Information

Alternative name: ClpB heat shock protein, N-terminal (HSP101)

Immunogen: recombinant Arabidopsis thaliana Hsp101/ClpB, N-terminal 145 amino acidsP42730

Format: Liquid

Description: Hsp101/ClpB is a member of HSP100 protein family. These proteins help proteinaggregates formed during heat stress to fall apart to allow them to be refolded byother chaperones. In spite of expression during heat stress members of HSP100protein family are also expressed during seed development.

Specificity: Predicted reactivity: dicots including: Glycine max, Nicotiana tabacum, Vitis vinifera.

Application Details

Application Notes: Recommended Dilution 1: 1000 with standard ECL (WB).

Buffer: total IgY in PBS pH 8.0+ 0.02% sodium azide

Storage: store at 4°C, make aliquots to avoid working with a stock. Please, remember tospin tubes briefly prior to opening them to avoid any losses that might occur fromliquid material adhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

From left to right: Arabidopsis thaliana WT grown at 22°C f (1), Arabidopsis thaliana WT stressed at 38°C/1.5h followed by a transfer to 22°C/2h (2), Arabidopsis thaliana Hsp101 null mutant grown at 22°C f (3), Arabidopsis Arabidopsis thaliana Hsp101 null mutant stressed at 38°C/1.5h followed by a transfer to 22°C/2h (4), 2 ug of a total protein loaded per well