Secretion-Associated and Ras-Related Protein 1 (Sar1) antibody

Antigen: Secretion-Associated and Ras-Related Protein 1 (Sar1)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Wheat (Triticum), Proso millet (Panicum miliaceum), Guinea gras (Panicum maximum), Barnyard Grass (Echinochloa crus-galli), Lovegrass (Eragrostis neomexicana), Large Crabgrass (Digitaria sanguinalis), Ferns

Application: Western Blotting (WB), Immunolocalization (IL)

Catalog no.: ABIN249384

Additional Information

Immunogen: GST fusion of full length recombinant Sar1 of Arabidopsis thaliana Q8VYP7

Format: Lyophilized

Description: Sar1 belongs to a small GTPase superfamily and GTP binging activity. Thisprotein is involved in intracellular protein transport. There are two different non-clathrin-coated vesicles that are responsible for transport between ER andthe Golgi. Coat protein complexes involving membrane-associated GTP bingingproteins – Arf1 and Sar1p for COPI and COPII are needed for formation ofCOP-coated vesicles. Sar1p is a cytosolic protein. It is temporarily recruited ontothe membranes of the ER.

Specificity: Predicted reactivity: dicots including Brassica campestris, Solanum tuberosum monocots includingTriticum aestivum, Oryza sativa, Zea mays, tree: Populus trichocarpa, mossPhyscomitrella patens. Not reactive in: Nicotiana tabacum.

Application Details

Application Notes: Recommended Dilution 1: 500 with standard ECL (WB), 1: 50 (IG). Additional Information: For immunogold experiments plant tissue has been fixed with GA in PFA/PIPES.LR White resin has been used. Tested species were: Triticum aestivum, Panicummiliaceum, Panicum maximum, Echinochloa crus-galli Eragrostis neomexicana,Digitaria sanguinalis. Publication in preparation.

Purity: Serum

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Publications

Publications: Pimpl, Movafeghi, Coughlan et al.: "In situ localization and in vitro induction of plant COPI-coated vesicles." in: The Plant cell, Vol. 12, Issue 11, pp. 2219-36, 2001 (PubMed).

Tnani, La: "Protein composition analysis of oil bodies from maize embryos during germination." in: Journal of plant physiology, 2011 (PubMed).