gamma Subunit, Cop Vesicles (Sec21P) antibody

Antigen: gamma Subunit, Cop Vesicles (Sec21P)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Maize/Corn (Zea mays)

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN249385

Additional Information

Immunogen: GST fusion of a part of recombinant Sec21 of Arabidopsis thaliana Q0WW26

Format: Lyophilized

Description: Sec21p is a constituent of the COPI vesicle coatomer.

Specificity: Predicted reactivity: dicots including Vitis vinifera, monocots inclusing Oryza sativa, Zea mays,deciduous broadleaf tree Populus trichocarpa. Not reactive in: Nicotiana tabacum.

Application Details

Application Notes: Recommended Dilution 1: 1000 with standard ECL (WB), 1: 1000 (IF).

Purity: Serum

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

From left to right: Nicotiana tabacum protoplast total protein with Triton X100, 0.02% (1), Arabidopsis thaliana protoplast soluble protein (2), Arabidopsis thaliana protoplast total protein with Triton X100 0.02% (3), (50 ug of total protein extracts from cell cultures were loaded per lane)

anti-gamma Subunit, Cop Vesicles (Sec21P) antibody (Image 2)

Publications

Publications: Movafeghi, Happel, Pimpl et al.: "Arabidopsis Sec21p and Sec23p homologs. Probable coat proteins of plant COP-coated vesicles." in: Plant physiology, Vol. 119, Issue 4, pp. 1437-46, 1999 (PubMed).