Fucose antibody

Antigen: Fucose

Clonality: Polyclonal

Host: Rabbit

Reactivity: Higher Plants

Application: Western Blotting (WB)

Catalog no.: ABIN249420

Additional Information

Immunogen: core fucose residues bound to the N-glycan in alpha 1,3

Format: Lyophilized

Description: This antibody specifically cross-reacts against fucose residues bound to theprotein N-glycans in alpha 1,3. This residue is characterisitc of the plant proteinN-glycans and is absent in protein N-glycans from animals. This residue is addedin the Golgi apparatus.

Specificity: Predicted reactivity: higher plants.

Application Details

Application Notes: Recommended Dilution 1 ug/10 ml of incubation buffer, with standard ECL (WB). Additional Information: Alpha (1,3) fucose is present not only in plants but also in some invertebrates(such as nematodes, bees, etc.) . However, cross-reaction with glycoproteins fromthese organisms is weaker than the one observed in plants. This sugar residuedoes not exist in mammals, in their endogenous glycoproteins.

Buffer: Affinity purified serum in PBS pH 7.4

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

From left to right: total cell extract from Arabidopsis thaliana wild type and cell extracts from different mutants defective in fucosyltransferases (data not published yet)