Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) antibody

Antigen: Insulin-Like Growth Factor Binding Protein 6 (IGFBP6)

Synonyms: IBP6, IGFBP-6, RBP6A, IGF-BP6, IGFBP6, MGC134265, igfbp6

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN263197

Additional Information

Alternative name: IGFBP6

Gene ID: 3489

Immunogen: C-RPQDVNRRDQQRNP

Format: Affinity-purified

Description: All names and symbols: IGFBP6 , insulin-like growth factor binding protein 6 , IBP6, IGF binding protein 6. Official Symbol: IGFBP6 . RefSeq number(s): NP_002169.1

Specificity: Human, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 25kDa band observed in Human Adrenal Gland lysates (calculated MW of 25.3kDa according to NP_002169.1). In transfected HEK293 transiently expressing IGFBP6 a band of approx. 29kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Diabetes, Metabolism, Growth Factors

Restrictions: For Research Use only

Images

ABIN263197 (0.5µg/ml) staining of Human Adrenal Gland lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing IGFBP6 (RC204060) and probed with ABIN263197 (mock transfection in first lane), tested by Origene.

Publications

Publications: Cheung, Vesey, Cotterill et al.: "Altered messenger RNA and protein expressions for insulin-like growth factor family members in clear cell and papillary renal cell carcinomas." in: International journal of urology : official journal of the Japanese Urological Association, Vol. 12, Issue 1, pp. 17-28, 2005 (PubMed).