X-Box Binding Protein 1 (XBP1) antibody

Antigen: X-Box Binding Protein 1 (XBP1)

Synonyms: XBP2, TREB5, XBP-1, TREB-5, D11Ertd39e, cb918, treb5, trebf, xbp1a, xbp1b, id:ibd1195, wu:fb02h05, wu:fb63f10, HTF, xbp1, MGC53678, MGC130816, xbp2, xXBP1, MGC76163, XBP1, XBPP1, MGC127699

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN263217

Additional Information

Alternative name: XBP1 / TREB5

Gene ID: 7494, 22433 (mouse)

Immunogen: C-NHSWEDTFANELFPQ

Format: Affinity-purified

Description: All names and symbols: XBP1, X-box binding protein 1 , TREB5, XBP2, OTTHUMP00000028682, X-box-binding protein-1. Official Symbol: XBP1. RefSeq number(s): NP_001073007.1

Specificity: This antibody is expected to recognise isoform XBP1(S) (NP_001073007.1) only. Human, Mouse, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 38kDa band observed in Nuclear lysates of cell line HeLa (calculated MW of 40.1kDa according to NP_001073007.1). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN263217 (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Yokouchi, Hiramatsu, Hayakawa et al.: "Involvement of selective reactive oxygen species upstream of proapoptotic branches of unfolded protein response." in: The Journal of biological chemistry, Vol. 283, Issue 7, pp. 4252-60, 2008 (PubMed).