Hypocretin (Orexin) Receptor 1/2 (HCRTR1/HCRTR2) antibody

Antigen: Hypocretin (Orexin) Receptor 1/2 (HCRTR1/HCRTR2)

Synonyms: OX1R, LOC443167, HCRTR1

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN263226

Additional Information

Alternative name: OX1R and OX2R

Gene ID: 3061, 31, 230777 (mouse)

Immunogen: C-YNFLSGKFREQFK

Format: Affinity-purified

Description: All names and symbols: OX1R, OX2R, HCRTR1, hypocretin (orexin) receptor 1, hypocretin receptor 1, hypocretin receptor-1, orexin receptor 1, orexin receptor-1, HCRTR2, hypocretin (orexin) receptor 2, hypocretin receptor 2, hypocretin receptor-2, orexin receptor 2, orexin receptor-2. Official Symbol: HCRTR1. RefSeq number(s): NP_001516.1, NP_001517.1

Specificity: Human, Mouse, Rat, Dog, Cow, Pig

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 48kDa band observed in Human Brain (Frontal Cortex), Mouse Brain and Rat Brain lysates (calculated MW of 47.5kDa according to NP_001516.1 and NP_001517.1). Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN263226 (0.5µg/ml) staining of Frontal Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Fukunaka, Shinkai, Hwang et al.: "The orexin 1 receptor (HCRTR1) gene as a susceptibility gene contributing to polydipsia-hyponatremia in schizophrenia." in: Neuromolecular medicine, Vol. 9, Issue 4, pp. 292-7, 2007 (PubMed).