OCT4 antibody
-
- Target See all OCT4 (POU5F1) Antibodies
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This OCT4 antibody is un-conjugated
-
Application
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- OCT-4 immunostaining has been shown to be a sensitive and specific marker for seminomatous / (dys)germinomatous tumours and in embryonal carcinoma variants of non-seminomatous GCTs, whether in primary gonadal or extragonadal sites or in metastatic lesions.
- Cross-Reactivity (Details)
- Species reactivity (tested):Human.
- Characteristics
- Synonyms: POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-bindingtranscription factor 3, Oct-3, Oct-4
- Purification
- Purified
- Immunogen
- BALB/C mice injected with synthetic peptide of human OCT-4 origin.
- Clone
- NRG1-1
- Isotype
- IgG2b
- Top Product
- Discover our top product POU5F1 Primary Antibody
-
anti-POU Class 5 Homeobox 1 (POU5F1) antibody
KO Validated POU5F1 Reactivity: Human WB, IF, ICC, IP, FACS, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibodyPOU5F1 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 1B11 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 201-300) antibodyPOU5F1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibodyPOU5F1 Reactivity: Human WB, ELISA, IHC, IF, FACS Host: Mouse Monoclonal 2H41D8 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) antibodyPOU5F1 Reactivity: Human WB, ELISA, ICC, FACS Host: Mouse Monoclonal 7E7 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 136-360) antibodyPOU5F1 Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibodyPOU5F1 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (Internal Region) antibodyPOU5F1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibodyPOU5F1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 1D2 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) antibodyPOU5F1 Reactivity: Human, Monkey WB, IF, IHC (p) Host: Rabbit Polyclonal unconjugated
-
- Application Notes
-
Suitable for Immunohistochemistry on Formalin fixed paraffin embedded tissue sections: 1/25-1/100 in an ABC method. Positive Control: Seminoma. Cellular Localization: Nuclear.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocol
- Staining ProtocolWe suggest an incubation period of 30 minutes at room temperature. However, dependingupon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require
- Restrictions
- For Research Use only
-
- Format
- Liquid
-
- Target
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
- Alternative Name
- OCT3/4 (POU5F1 Products)
- Background
- OCT-4 (also known as POU5F1), a transcription factor that has been recognized as fundamental in the maintenance of pluripotency in embryonic stem cells and primordial germ cells, has been proposed as a useful marker for Germ Cell Tumors (GCTs) that exhibit features of pluripotentiality, specifically seminoma / dysgerminoma / germinoma and embryonal carcinoma.Synonyms: OCT3, OCT4, OTF3, Oct-3, Oct-4, Octamer-binding transcription factor 3, POU domain, POU5F1, class 5, transcription factor 1
- Gene ID
- 5460
- UniProt
- Q01860
- Pathways
- Stem Cell Maintenance
-
