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Details for Product No. ABIN263989

POU Class 5 Homeobox 1 (POU5F1) antibody

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Antigen
Synonyms OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, NF-A3, Oct-3/4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4, POU5F1, POU5F1L
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 3 references available
Catalog no. ABIN263989
Quantity 0.5 mL
Price
390.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days

Product details

Target Details Application Details Handling References Alternatives back to top
Immunogen BALB/C mice injected with synthetic peptide of human OCT-4 origin.
Clone NRG1-1
Isotype IgG2b
Characteristics Synonyms: POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-bindingtranscription factor 3, Oct-3, Oct-4
Purification Purified

Target Details

Application Details Handling References Alternatives back to top
Alternative Name OCT3/4
Background OCT-4 (also known as POU5F1), a transcription factor that has been recognized asfundamental in the maintenance of pluripotency in embryonic stem cells and primordialgerm cells, has been proposed as a useful marker for Germ Cell Tumors (GCTs) that exhibitfeatures of pluripotentiality, specifically seminoma / dysgerminoma / germinoma andembryonal carcinoma.
UniProt Q01860

Application Details

Target Details Handling References Alternatives back to top
Application Notes Suitable for Immunohistochemistry on Formalin fixed paraffin embedded tissue sections: 1/25-1/100 in an ABC method. Positive Control: Seminoma. Cellular Localization: Nuclear. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Staining ProtocolWe suggest an incubation period of 30 minutes at room temperature. However, dependingupon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require
Restrictions For Research Use only

References

Target Details Application Details Handling Alternatives back to top
Background publications Hattab, Tu, Wilson et al.: "OCT4 immunohistochemistry is superior to placental alkaline phosphatase (PLAP) in the diagnosis of central nervous system germinoma." in: The American journal of surgical pathology, Vol. 29, Issue 3, pp. 368-71, 2005 (PubMed).

Lau, Chang: "OCT4: a sensitive and specific immunohistochemical marker for metastatic germ cell tumors." in: Advances in anatomic pathology, Vol. 13, Issue 2, pp. 76-9, 2006 (PubMed).

Jones, MacLennan, Bonnin et al.: "Screening for intratubular germ cell neoplasia of the testis using OCT4 immunohistochemistry." in: The American journal of surgical pathology, Vol. 30, Issue 11, pp. 1427-31, 2006 (PubMed).

Alternatives

Target Details Application Details Handling References back to top
Alternatives for antigen "POU Class 5 Homeobox 1 (POU5F1)", type "Antibodies"
Hosts (142), (61), (12), (11)
Reactivities (200), (81), (45), (35), (30), (30), (11), (3), (3), (2), (2), (2), (2), (2), (1)
Applications (162), (79), (44), (28), (24), (20), (20), (15), (7), (6), (6), (3), (1), (1)
Conjugates (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Epitopes (17), (15), (10), (7), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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