Human Adenovirus type 2 E1A (HAdV-2 E1A) antibody
This Mouse Monoclonal antibody specifically detects Human Adenovirus type 2 E1A in IP and IF. It exhibits reactivity toward Human Adenovirus 2.
Catalog No. ABIN264328
$1,002.00
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0.1 mg
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United States
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Quick Overview for Human Adenovirus type 2 E1A (HAdV-2 E1A) antibody (ABIN264328)
Target
Human Adenovirus type 2 E1A (HAdV-2 E1A)
Reactivity
Human Adenovirus 2
Host
Mouse
Clonality
Monoclonal
Conjugate
Un-conjugated
Application
Immunoprecipitation (IP), Immunofluorescence (IF)
Clone
M73
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Characteristics
- Synonyms: Adenovirus Early Region 1, Early E1A 32 kDa protein
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Purification
- Purified
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Immunogen
- Full length recombinant adenovirus 2 E1A protein.
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Isotype
- IgG2a
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Application Notes
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Immunoprecipitation (1-2 μg/mL): Adenovirus 2E1A recognizes adenovirus 2 and 5 E1Aproteins, 3 bands at 30-50 kDa, as well as, E1A associated proteins. Immunofluorescence: 2-5 μg/mL.293 cells can be used as positive control and HS27 cells as negative control. Positive Control: 293 cells are human embryonic kidney cells that have been transformedwith the adenovirus genes E1A and E1B, which are required for adenovirus propagation. This cell line can be used as a positive control for this antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Buffer
- PBS containing 0.08 % Sodium Azide as preservative.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
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Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. -
Expiry Date
- 12 months
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- Human Adenovirus type 2 E1A (HAdV-2 E1A)
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Alternative Name
- Adenovirus Type 2 E1A
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Target Type
- Viral Protein
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Background
- The early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11 % of the viral genome and consists of two transcriptional units E1A and E1B. E1A is sufficient for partial transformation and immortalization of primary Cells. E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection and are able to either activate or repress the transcription of specific cellular genes. E1A forms specific complexes with cellular proteins including p105 causing inhibition of the cell cycle inducing arresting function of p105.Synonyms: Adenovirus Early Region 1, Early E1A 32 kDa protein
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UniProt
- P03254
Target
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