Green Fluorescent Protein (GFP) antibody

Details for Product No. ABIN264457
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Antigen
Reactivity
Aequorea victoria
(341), (31), (11), (4), (3), (3), (2), (1), (1)
Host
Mouse
(148), (146), (56), (26), (4), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(24), (19), (14), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(300), (191), (107), (62), (59), (47), (38), (29), (29), (16), (13), (10), (8), (4), (3), (2)
Pubmed 3 references available
Catalog no. ABIN264457
Quantity 0.1 mg
Price
324.50 $   Plus shipping costs $45.00
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Immunogen Hybridoma produced by the fusion of splenocytes of mice immunized with full-length greenfluorescent protein and mouse myeloma cells.
Clone F56-BA1-2-3
Isotype IgG
Characteristics Synonyms: Green fluorescent protein, GFP-Tag
Purification Purified
Alternative Name GFP
Background Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequoreavictoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited byblue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) hasbecome an invaluable tool in cell biology research, since its intrinsic fluorescence can bevisualized in living cells. GFP fluorescence is stable under fixation conditions and suitablefor a variety of applications. GFP has been widely used as a reporter for gene expression,enabling researchers to visualize and localize GFP-tagged proteins within living cellswithout the need for chemical staining. Other applications of GFP include assessment ofprotein protein interactions through the yeast two hybrid system and measurement ofdistance between proteins through fluorescence energy transfer (FRET) protocols. GFPtechnnology has considerably contributed to a greater understanding of cellularphysiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFPshould also detect YFP and other variants.
UniProt P42212
Research Area Tags/Labels
Application Notes Western Blot (5-10 ug/ml). Positive Control: Purified Green Fluorescent Protein. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing containing 0.08% Sodium Azide as preservative.
Preservative Sodium azide
Storage -20 °C
Storage Comment Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Expiry Date 12 months
Background publications Prasher, Eckenrode, Ward et al.: "Primary structure of the Aequorea victoria green-fluorescent protein." in: Gene, Vol. 111, Issue 2, pp. 229-33, 1992 (PubMed).

Inouye, Tsuji: "Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein." in: FEBS letters, Vol. 341, Issue 2-3, pp. 277-80, 1994 (PubMed).

Cody, Prasher, Westler et al.: "Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein." in: Biochemistry, Vol. 32, Issue 5, pp. 1212-8, 1993 (PubMed).

Hosts (148), (146), (56), (26), (4), (2), (1)
Reactivities (341), (31), (11), (4), (3), (3), (2), (1), (1)
Applications (300), (191), (107), (62), (59), (47), (38), (29), (29), (16), (13), (10), (8), (4), (3), (2)
Conjugates (24), (19), (14), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (26), (18), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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