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GFP antibody

Name: GFP antibody
SKU: ABIN2451988
Price: 430 USD
Availability: InStock

GFP Reactivity: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq Host: Rat Monoclonal 1A5 unconjugated

Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN2451988)

Immunofluorescence (IF) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN2451988)

SDS-PAGE (SDS) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN2451988)

SDS

Immunoprecipitation (IP) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN2451988)

7 images

(1 reference)

Catalog No. ABIN2451988

Datasheet as PDF

Target See all GFP Antibodies

GFP (Green Fluorescent Protein (GFP))
Reactivity
174

19

15

15

12

7

5

4

2

1

1

1

1
Aequorea victoria
Host
74

73

35

11

9

6

1

1
Rat
Compatible Secondaries
Clonality
121

84
Monoclonal
Conjugate
103

14

12

9

6

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
Application
172

86

55

42

32

31

29

21

16

14

14

14

12

5

4

4

3

3

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunochromatography (IC), ChIP DNA-Sequencing (ChIP-seq)

Specificity

Specific to GFP and GFP-fused proteins

Characteristics

The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.

Purification

Purified

Sterility

Sterile filtered

Immunogen

Recombinant GFP protein

Clone

1A5

Isotype

IgG1 kappa

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Application Notes

1. Western blotting: ~1 g/mL
2. Immunoprecipitation
3. Immunocytochemistry
4. Chromatin Immunoprecipitation (ChIP)
5. ELISA

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

PBS, 50 % glycerol

Preservative

Azide free

Storage

-20 °C/-80 °C

Storage Comment

-20 C (For long term storage: -80 C)
SHIMOMURA, JOHNSON, SAIGA: "Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea." in: Journal of cellular and comparative physiology, Vol. 59, pp. 223-39 (PubMed).
Target

GFP (Green Fluorescent Protein (GFP))

Alternative Name

GFP (GFP Products)

Synonyms

green fluorescent protein antibody, gfp antibody

Target Type

Viral Protein

Background

The green fluorescent protein (GFP) is composed of 238 amino acids (26.9 kDa), originally isolated from the jellyfish Aequorea victoria that fluoresces green when exposed to blue light. In cell and molecular biology, the GFP fused gene is frequently used as a reporter of expression and protein localization.

UniProt

P42212