GFP antibody

Catalog No. ABIN2451988

This Rat Monoclonal antibody specifically detects GFP in WB, ELISA, IP, IC and ChIP-seq. It exhibits reactivity toward Aequorea victoria.It has been mentioned in 1 publication

Quick Overview for GFP antibody (ABIN2451988)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Rat

Clonality: Monoclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunochromatography (IC), ChIP DNA-Sequencing (ChIP-seq)

Clone: 1A5

Product Details anti-GFP Antibody

Specificity: Specific to GFP and GFP-fused proteins

Characteristics: The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.

Purification: Purified

Sterility: Sterile filtered

Immunogen: Recombinant GFP protein

Isotype: IgG1 kappa

Application Details

Application Notes: 1. Western blotting: ~1 g/mL
2. Immunoprecipitation
3. Immunocytochemistry
4. Chromatin Immunoprecipitation (ChIP)
5. ELISA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Preservative: Azide free

Storage: -20 °C/-80 °C

Storage Comment: -20 C (For long term storage: -80 C)

References for anti-GFP antibody (ABIN2451988)

SHIMOMURA, JOHNSON, SAIGA: "Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea." in: Journal of cellular and comparative physiology, Vol. 59, pp. 223-39 (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: The green fluorescent protein (GFP) is composed of 238 amino acids (26.9 kDa), originally isolated from the jellyfish Aequorea victoria that fluoresces green when exposed to blue light. In cell and molecular biology, the GFP fused gene is frequently used as a reporter of expression and protein localization.

UniProt: P42212