- Key Features
- Polyclonal, unconjugated GFP antibody for reliable detection of GFP and its variants.
- Validated for Fluorescence Microscopy, ELISA, Western Blotting
- High Quality GFP antibody, cited in more than 177 PubMed References.
- Available in 10 µl and 100 µl quantities.
- Target See all GFP Antibodies
- GFP (Green Fluorescent Protein (GFP))
- Aequorea victoria
- This GFP antibody is un-conjugated
- Fluorescence Microscopy (FM), ELISA, Western Blotting (WB)
- Goat Anti-GFP is ideal for western blotting, ELISA, Immunohistochemistry and IP.
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.
- Cross-Reactivity (Details)
- wt, rGFP, eGFP
- Anti-GFP is designed to detect GFP and its variants.
- GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Sterile filtered
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
- Product Specific Information
What can the GFP antibody ABIN100085 be used for? This unconjugated, polyclonal GFP antibody has been developed to detect GFP and derivatives. It reliably detects recombinant and enhanced variants of the protein in addition to wild-type GFP. The Goat GFP antibody has been validated for Western blot, immunofluorescence microscopy and ELISA. Use our GFP antibody to detect GFP by direct antigen binding with sandwich or capture ELISA. Detect GFP by fluorescence microscopy in prokaryotic and eukaryotic expression systems, for example in E.coli or CHO cells. Use a fluorochrome conjugate for this purpose, which again leads to a significant enhancement of the signal provided by the natural fluorescence of GFP. For immunoblotting, use either peroxidase or alkaline phosphatase conjugated polyclonal GFP antibody to detect GFP or GFP-containing proteins on Western blots.
What validation data is available for this GFP antibody? The GFP antibody has been used in more than 177 publications to date, which are listed below and can be viewed at Pubmed. More than 14 Product images illustrate performance in western blot and fluorescence microscopy.
What is the function of GFP? GFP, the green fluorescent protein, is a fluorescent protein originally isolated from the jellyfish Aequorea victoria. It emits green light of wavelength 509nm when excited with blue light. GFP is widely used in cell research. It allows proteins labeled with GFP to be visualized in living cells, allowing a wide range of investigations.
anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea aequorea IF, IHC (fro), IHC (p), WB Host: Goat Polyclonal unconjugatedanti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria ChIP-seq, IC, IP, ELISA, WB Host: Rat Monoclonal 1A5 unconjugatedanti-Green Fluorescent Protein (GFP) antibody
GFP ELISA, FACS, IF, IP, WB Host: Mouse Monoclonal 6C11C11 unconjugatedanti-Green Fluorescent Protein (GFP) (AA 246) antibody
GFP Reactivity: Aequorea victoria ELISA, WB Host: Mouse Monoclonal 9F9-F9 unconjugatedanti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria IP, ELISA, WB Host: Rabbit Polyclonal unconjugatedanti-Green Fluorescent Protein (GFP) (AA 246) antibody
GFP Reactivity: Aequorea victoria FM, ELISA, WB Host: Rabbit Polyclonal unconjugatedanti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria FACS, IHC, ELISA, WB Host: Mouse Monoclonal 4B10B2 unconjugatedanti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria IEM, IF, IHC (fro), IHC (p), WB Host: Goat Polyclonal unconjugatedanti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)
GFP Reactivity: Aequorea victoria FACS, IM Host: Goat Polyclonal FITCanti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: General FACS, IHC, ELISA, WB Host: Mouse Monoclonal unconjugated
- Application Notes
ELISA : 1:10,000 - 1:30,000
IF Microscopy : 1:500
Western Blot : 1:1,000 - 1:10,000
Immunohistochemistry: 1:200 - 1:1,000
This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (ABIN129564) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin-HRP (ABIN964537).
Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and detects GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone.
For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP-containing proteins on western blots. Researchers should determine optimal titers for applications.
- For Research Use only
- 1.0 mg/mL
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid cycles of freezing and thawing.
- -20 °C
- Storage Comment
- Store GFP antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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: "An Interscholastic Network To Generate LexA Enhancer Trap Lines in Drosophila. ..." in: G3 (Bethesda, Md.), Vol. 9, Issue 7, pp. 2097-2106, (2020) (PubMed).
: "Local but not systemic administration of mesenchymal stromal cells ameliorates fibrogenesis in regenerating livers." in: Journal of cellular and molecular medicine, Vol. 23, Issue 9, pp. 6238-6250, (2020) (PubMed).
: "An Adeno-Associated Virus-Based Toolkit for Preferential Targeting and Manipulating Quiescent Neural Stem Cells in the Adult Hippocampus." in: Stem cell reports, Vol. 10, Issue 3, pp. 1146-1159, (2019) (PubMed).
- Asymmetric activity of NetrinB controls laterality of the Drosophila brain." in: Nature communications, Vol. 14, Issue 1, pp. 1052, (2023) (PubMed).
- GFP (Green Fluorescent Protein (GFP))
- Alternative Name
- GFP (GFP Products)
- green fluorescent protein antibody, gfp antibody
- Target Type
- Viral Protein
Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.