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GFP antibody (Green Fluorescent Protein) Primary Antibody

GFP Reactivity: Aequorea victoria FM, ELISA, WB Host: Goat Polyclonal unconjugated
Pubmed (175)
Catalog No. ABIN100085
$295.00
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100 μL ABIN100085
100 μL ABIN100085
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  • Target
    GFP
    Reactivity
    Aequorea victoria
    • 189
    • 7
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Host
    Goat
    • 77
    • 76
    • 31
    • 16
    • 12
    • 7
    • 3
    • 1
    Clonality
    Polyclonal
    • 129
    • 90
    Conjugate
    This GFP antibody is un-conjugated
    • 121
    • 11
    • 10
    • 10
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Application
    Fluorescence Microscopy (FM), ELISA, Western Blotting (WB)
    • 156
    • 92
    • 49
    • 47
    • 37
    • 36
    • 32
    • 21
    • 14
    • 12
    • 10
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Purpose
    Goat Anti-GFP is ideal for western blotting, ELISA, Immunohistochemistry and IP.
    Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.
    Cross-Reactivity (Details)
    wt, rGFP, eGFP
    Characteristics
    Anti-GFP is designed to detect GFP and its variants.
    Purification
    GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    Sterility
    Sterile filtered
    Immunogen
    The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
    Immunogentype: Recombinant
    Isotype
    IgG
  • Application Notes
    ELISA : 1:10,000 - 1:30,000
    IF Microscopy : 1:500
    Western Blot : 1:1,000 - 1:10,000
    Immunohistochemistry: 1:200 - 1:1,000
    ImmunoPrecipitation: Yes
    Comment

    This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (ABIN129564) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin-HRP (ABIN964537).
    Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and detects GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone.
    For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP-containing proteins on western blots. Researchers should determine optimal titers for applications.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid cycles of freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store GFP antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
  • Cone, Hurwitz, Lee, Yuan, Zhou, Li, Meckes: "Alix and Syntenin-1 direct amyloid precursor protein trafficking into extracellular vesicles." in: BMC molecular and cell biology, Vol. 21, Issue 1, pp. 58, 2020 (PubMed).

    Kim, Kim, Choi, Lee, Lee, Im, Shin, Kim, Hong, Kim, Kim, Sung: "Downregulated miR-18b-5p triggers apoptosis by inhibition of calcium signaling and neuronal cell differentiation in transgenic SOD1 (G93A) mice and SOD1 (G17S and G86S) ALS patients." in: Translational neurodegeneration, Vol. 9, Issue 1, pp. 23, 2020 (PubMed).

    Saegusa, Hosoya, Nishiyama, Saeki, Fujimoto, Okano, Fujioka, Ogawa: "Low-dose rapamycin-induced autophagy in cochlear outer sulcus cells." in: Laryngoscope investigative otolaryngology, Vol. 5, Issue 3, pp. 520-528, 2020 (PubMed).

    Nkosi, Sun, Duke, Patel, Surapaneni, Singh, Meckes: "Epstein-Barr Virus LMP1 Promotes Syntenin-1- and Hrs-Induced Extracellular Vesicle Formation for Its Own Secretion To Increase Cell Proliferation and Migration." in: mBio, Vol. 11, Issue 3, 2020 (PubMed).

    Rayaprolu, Gao, Xiao, Ramesha, Weinstock, Shah, Duong, Dammer, Webster, Lah, Wood, Betarbet, Levey, Seyfried, Rangaraju: "Flow-cytometric microglial sorting coupled with quantitative proteomics identifies moesin as a highly-abundant microglial protein with relevance to Alzheimer's disease." in: Molecular neurodegeneration, Vol. 15, Issue 1, pp. 28, 2020 (PubMed).

    Lee, Hanchate, Kondoh, Tong, Kuang, Spray, Ye, Buck: "A psychological stressor conveyed by appetite-linked neurons." in: Science advances, Vol. 6, Issue 12, pp. eaay5366, 2020 (PubMed).

    Chen, Mohammad, Pazdernik, Huang, Bowman, Tycksen, Schedl: "GLP-1 Notch-LAG-1 CSL control of the germline stem cell fate is mediated by transcriptional targets lst-1 and sygl-1." in: PLoS genetics, Vol. 16, Issue 3, pp. e1008650, 2020 (PubMed).

    Li, Bao, Luo, Yoan, Sullivan, Quintanilla, Wickersham, Lazarus, Shin, Song: "Supramammillary nucleus synchronizes with dentate gyrus to regulate spatial memory retrieval through glutamate release." in: eLife, Vol. 9, 2020 (PubMed).

    Schäfer, Kaisler, Scheller, Kirchhoff, Haghikia, Faissner: "Conditional Deletion of LRP1 Leads to Progressive Loss of Recombined NG2-Expressing Oligodendrocyte Precursor Cells in a Novel Mouse Model." in: Cells, Vol. 8, Issue 12, 2020 (PubMed).

    Lehner, Spitzer, Gehwolf, Wagner, Weissenbacher, Deininger, Emmanuel, Wichlas, Tempfer, Traweger: "Tenophages: a novel macrophage-like tendon cell population expressing CX3CL1 and CX3CR1." in: Disease models & mechanisms, Vol. 12, Issue 12, 2020 (PubMed).

    Ganjam, Bolte, Matschke, Neitemeier, Dolga, Höllerhage, Höglinger, Adamczyk, Decher, Oertel, Culmsee: "Mitochondrial damage by α-synuclein causes cell death in human dopaminergic neurons." in: Cell death & disease, Vol. 10, Issue 11, pp. 865, 2020 (PubMed).

    Bongartz, Gille, Hessenkemper, Mandel, Lewitzky, Feller, Schaper: "The multi-site docking protein Grb2-associated binder 1 (Gab1) enhances interleukin-6-induced MAPK-pathway activation in an SHP2-, Grb2-, and time-dependent manner." in: Cell communication and signaling : CCS, Vol. 17, Issue 1, pp. 135, 2020 (PubMed).

    Javier-Torrent, Marco, Rocandio, Pons-Vizcarra, Janes, Lackmann, Egea, Saura: "Presenilin/γ-secretase-dependent EphA3 processing mediates axon elongation through non-muscle myosin IIA." in: eLife, Vol. 8, 2020 (PubMed).

    Shibata, Iseda, Mitsuhashi, Oka, Shindo, Moritoki, Nagai, Otsubo, Inoue, Sasaki, Akazawa, Takahashi, Schalek, Lichtman, Okano: "Large-Area Fluorescence and Electron Microscopic Correlative Imaging With Multibeam Scanning Electron Microscopy." in: Frontiers in neural circuits, Vol. 13, pp. 29, 2020 (PubMed).

    Turnham, Smith, Kenerson, Omar, Golkowski, Garcia, Bauer, Lau, Sullivan, Langeberg, Ong, Riehle, Yeung, Scott: "An acquired scaffolding function of the DNAJ-PKAc fusion contributes to oncogenic signaling in fibrolamellar carcinoma." in: eLife, Vol. 8, 2020 (PubMed).

    Zibetti, Liu, Wan, Qian, Blackshaw: "Epigenomic profiling of retinal progenitors reveals LHX2 is required for developmental regulation of open chromatin." in: Communications biology, Vol. 2, pp. 142, 2020 (PubMed).

    Kockel, Griffin, Ahmed, Fidelak, Rajan, Gould, Haigney, Ralston, Tercek, Galligani, Rao, Huq, Bhargava, Dooner, Lemmerman, Malusa, Nguyen, Chung, Gregory, Kuwana, Regenold, Wei, Ashton, Dickinson et al.: "An Interscholastic Network To Generate LexA Enhancer Trap Lines in Drosophila. ..." in: G3 (Bethesda, Md.), Vol. 9, Issue 7, pp. 2097-2106, 2020 (PubMed).

    Crowther, Lim, Asrican, Albright, Wooten, Yeh, Bao, Cerri, Hu, Ian Shih, Asokan, Song: "An Adeno-Associated Virus-Based Toolkit for Preferential Targeting and Manipulating Quiescent Neural Stem Cells in the Adult Hippocampus." in: Stem cell reports, Vol. 10, Issue 3, pp. 1146-1159, 2019 (PubMed).

    Kaczmarek-Hajek, Zhang, Kopp, Grosche, Rissiek, Saul, Bruzzone, Engel, Jooss, Krautloher, Schuster, Magnus, Stadelmann, Sirko, Koch-Nolte, Eulenburg, Nicke: "Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody." in: eLife, Vol. 7, 2019 (PubMed).

    Neubauer, Tea, Zebol, Gliddon, Stefanidis, Moretti, Pitman, Costabile, Kular, Stringer, Day, Samuel, Bonder, Powell, Pitson: "Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma." in: Oncogene, Vol. 38, Issue 8, pp. 1151-1165, 2019 (PubMed).

  • Target
    GFP
    Alternative Name
    GFP
    Synonyms
    green fluorescent protein, gfp
    Background
    Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
    Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.
    UniProt
    P42212
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