GFP antibody

Catalog No. ABIN6254149

This Goat Polyclonal antibody specifically detects GFP in WB, IF, IHC (fro), IHC (p) and IEM. It exhibits reactivity toward Aequorea victoria.

Quick Overview for GFP antibody (ABIN6254149)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Goat

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoelectron Microscopy (IEM)

Product Details anti-GFP Antibody

Purpose: anti-Venus

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody also recognizes Venus/GFP and does not cross-react to mCherry/red fluorescent proteins.

Cross-Reactivity (Details): YFP, GFP, EGFP, Venus

Characteristics: Venus Fluorescent Protein

Purification: Epitope affinity purified

Immunogen: Purified recombinant GFP peptide produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2,000, IF:1:50-1:1,000, IHC-P:1:50-1:1,000, IHC-F:1:50-1:1,000, IEM:1:50-1:1,000

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: Venus

Background:

Goat polyclonal antibody to Venus. Venus is a genetic mutant of green fluorescent protein (GFP), derived from Aequorea victoria. Its excitation/emission peaks are 515/528 nm respectively and is often used as a donor-acceptor pair by researchers in FRET based co-localization studies.

GFP, Green fluorescent protein antibody.