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GFP antibody (Green Fluorescent Protein) Primary Antibody

GFP Reactivity: Aequorea victoria ICC, IP, WB Host: Rabbit Polyclonal unconjugated
Pubmed (2)
Catalog No. ABIN302066
$367.36
Plus shipping costs $45.00
0.1 mg
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  • Target See all GFP Antibodies
    GFP
    Reactivity
    • 187
    • 18
    • 15
    • 15
    • 6
    • 5
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Aequorea victoria
    Host
    • 75
    • 72
    • 32
    • 15
    • 10
    • 6
    • 3
    • 1
    Rabbit
    Clonality
    • 124
    • 86
    Polyclonal
    Conjugate
    • 118
    • 12
    • 11
    • 10
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GFP antibody is un-conjugated
    Application
    • 154
    • 97
    • 46
    • 44
    • 35
    • 33
    • 29
    • 22
    • 15
    • 15
    • 14
    • 12
    • 11
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
    Specificity
    The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in all species.
    Cross-Reactivity (Details)
    Recognizes fusion proteins in all species
    Purification
    Purified from rabbit serum by affinity chromatography
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    EGFP, a native full-length protein
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
  • Porrero, Rubio-Garrido, Avendauno, Clascua: "Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice." in: Brain research, 2010 (PubMed).

    Valenta, Lukas, Doubravska, Fafilek, Korinek: "HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies." in: The EMBO journal, Vol. 25, Issue 11, pp. 2326-37, 2006 (PubMed).

  • Target
    GFP
    Alternative Name
    GFP (GFP Products)
    Synonyms
    green fluorescent protein, gfp
    Target Type
    Viral Protein
    Background
    Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (λ,=509 nm) when excited (excitation by Blue or UV light, absorption peak λ,=395 nm). GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chomophore mutations have been created. The EnhancedGFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.
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