Adenovirus (0) antibody

Antigen: Adenovirus (0)

Clonality: Polyclonal

Host: Goat

Application: Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN264532

Additional Information

Characteristics: Synonyms: Adenovirus antibody

Alternative name: Adenovirus

Immunogen: Disrupted type 5 virions

Description: Adenoviruses are DNA viruses generally widespread in nature that are frequently the causeof acute upper respiratory tract infections (i. e. common colds). Forty-seven knownserotypes have been isolated since they were first discovered in 1953 with 3 types knownto cause gastroenteritis. Several types have oncogenic potential though most causeself-limiting febrile illnesses characterised by inflammation of conjunctivae and therespiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgicallyremoved, indicating latent infections. It is not known how long the virus can persist in thebody, or whether it is capable of reactivation after long periods. In patients experiencingimmunosuppression (e. g. AIDS) it can be reactivated causing disease. The adenovirus early gene products E1A is a potent stimulator of cellular proliferation,which when overexpressed can overcome the growth inhibitory effects of TGF beta. The E1Aregion encodes a series of related proteins (35 - 46 kD) with multifunctional capabilitiesand forms a specific complex with the retinoblastoma tumor suppressor gene product. TheE1a and E1B regions together comprise the transforming region of adenovirus. Whileexpression of E1A alone is sufficient to immortalize primary cells, complete transformationrequires the additional expression of the E1B region.

Specificity: This Adenovirus antibody reacts to Numerous Adenovirus proteins including hexon. Reacts with Adenovirus types 2, 3, 4, 5 and 6. Does not crossreact with Para 1-3, Influenza A & B, RSV, human MPV & Rhinovirus (type 16). Negative against HEp-2, LLCMK2 and Vero cells by indirect immunofluorescence. Add. Information: Centrifuge before opening to ensure complete recovery of vial contents.

Application Details

Application Notes: ELISA. IFA. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 4-5mg/ml (OD280nm, E0.1% = 1.4)

Purification: Purified

Buffer: 0.01M PBS, pH 7.2 containing 0,09% sodium azide

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Virology

Restrictions: For Research Use only