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Details for Product No. ABIN264873

V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody

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Antigen
Synonyms HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA, cb945, 2410016A19Rik, AW554154, PKB, PKBbeta, akt2-a, pkbb, pkbbeta, pkbss, prkbb, rac-beta, v-akt2, AKT2
Reactivity
»Alternatives Cow (Bovine), Dog (Canine), Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 3 references available
Catalog no. ABIN264873
Quantity 0.4 mL
Price
335.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen A five residue synthetic peptide based on the Human Akt2, coupled to KLH
Isotype IgG
Specificity This antibody detects a ~65kDa protein, corresponding to the molecular mass of Akt2 PKB beta on SDSPAGE immunoblots. Does not react with PKB-alpha (Akt1) or PKB-gamma (Akt3).
Purification Affinity Chromatography
Alternative Name AKT2 / PKB beta
Background The serine/threonine kinase Akt (protein kinase B or PKB) has a central role in the regulation of several signaling pathways controlling cell proliferation, apoptosis, angiogenesis, and diabetes. In humans, there are three genes in the "Akt family": Akt1, Akt2, and Akt3. AKT2 is a putative oncogene and is a general protein kinase capable of phophorylating several known proteins. AKT2 is amplified and overexpressed in some human carcinomas. AKT2 acts primarily as a regulator of glucose metabolism.
Alternate names: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine- protein kinase
Gene ID 208
NCBI Accession NP_001617.1
UniProt P31751
Research Area Signaling
Application Notes Western blot (3): 1: 13000 dilution of SPC-148 was ufficient for detection of PKBβ in 20 μg ofHeLa cell lysate by ECL immunoblot analysis. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer TBS 0.09% Sodium Azide 50% Glycerol
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Downward: "Mechanisms and consequences of activation of protein kinase B/Akt." in: Current opinion in cell biology, Vol. 10, Issue 2, pp. 262-7, 1998 (PubMed).

Brazil, Hemmings: "Ten years of protein kinase B signalling: a hard Akt to follow." in: Trends in biochemical sciences, Vol. 26, Issue 11, pp. 657-64, 2001 (PubMed).

Nicholson, Anderson: "The protein kinase B/Akt signalling pathway in human malignancy." in: Cellular signalling, Vol. 14, Issue 5, pp. 381-95, 2002 (PubMed).

Alternatives for antigen "V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2)", type "Antibodies"
Hosts (147), (47), (7), (3), (2)
Reactivities (205), (98), (88), (21), (20), (19), (19), (10), (2), (1), (1), (1), (1), (1), (1)
Applications (168), (84), (57), (48), (41), (40), (20), (15), (6), (4), (2), (1), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Epitopes (43), (13), (10), (9), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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