V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody

Details for Product No. ABIN264873
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Antigen
Synonyms AKT2, pkbb, pkbbeta, pkbss, prkbb, rac-beta, v-akt2, 2410016A19Rik, AW554154, PKB, PKBbeta, HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA, cb945, akt2-a
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
(248), (89), (75), (11), (6), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(178), (63), (10), (2), (2), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(8), (8), (8), (7), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
(236), (120), (101), (45), (39), (33), (15), (12), (12), (10), (10), (9), (3), (1)
Pubmed 3 references available
Quantity 0.4 mL
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Catalog No. ABIN264873
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Immunogen A five residue synthetic peptide based on the Human Akt2, coupled to KLH
Isotype IgG
Specificity This antibody detects a ~65 kDa protein, corresponding to the Molecular Mass of Akt2 PKB beta on SDS-PAGE immunoblots. Does not react with PKB-alpha (Akt1) or PKB-gamma (Akt3).
Purification Affinity Chromatography
Alternative Name AKT2 / PKB beta
Background Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).Synonyms: Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase
Gene ID 208
NCBI Accession NP_001617
UniProt P31751
Research Area Signaling
Application Notes Western blot (3): 1: 13000 dilution of SPC-148 was ufficient for detection of PKBβ in 20 μg ofHeLa cell lysate by ECL immunoblot analysis. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer TBS, 0.09 % Sodium Azide, 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody anti-V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) antibody
Background publications Downward: "Mechanisms and consequences of activation of protein kinase B/Akt." in: Current opinion in cell biology, Vol. 10, Issue 2, pp. 262-7, 1998 (PubMed).

Brazil, Hemmings: "Ten years of protein kinase B signalling: a hard Akt to follow." in: Trends in biochemical sciences, Vol. 26, Issue 11, pp. 657-64, 2001 (PubMed).

Nicholson, Anderson: "The protein kinase B/Akt signalling pathway in human malignancy." in: Cellular signalling, Vol. 14, Issue 5, pp. 381-95, 2002 (PubMed).

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