Phosphothreonine antibody (HRP)

Details for Product No. ABIN264876
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Antigen
Reactivity
Chemical
(43), (7), (5), (5), (4), (4)
Host
Rabbit
(40), (16)
Clonality
Polyclonal
Conjugate
HRP
(7), (7)
Application
ELISA, Western Blotting (WB)
(50), (49), (25), (7), (2), (2), (2), (1), (1), (1)
Pubmed 5 references available
Catalog no. ABIN264876
Quantity 0.4 mL
Price
456.50 $   Plus shipping costs $45.00
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Immunogen Phosphothreonine conjugated to KLH
Isotype IgG
Specificity This antibody recognizes proteins phosphorylated on Threonine residues. Slightly cross-reacts with phosphoserine but does not cross-react with Phosphotyrosine.
Purification Affinity Chromatography
Background Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications
Application Notes Western blot (5). ELISA (5). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS 0.09% Sodium Azide 50% Glycerol
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Ostrovsky, Maloy: "Protein phosphorylation on serine, threonine, and tyrosine residues modulates membrane-protein interactions and transcriptional regulation in Salmonella typhimurium." in: Genes & development, Vol. 9, Issue 16, pp. 2034-41, 1995 (PubMed).

Pawson, Saxton: "Signaling networks--do all roads lead to the same genes?" in: Cell, Vol. 97, Issue 6, pp. 675-8, 1999 (PubMed).

Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).

Downward: "The ins and outs of signalling." in: Nature, Vol. 411, Issue 6839, pp. 759-62, 2001 (PubMed).

Goto, Kiyono, Tomono et al.: "Complex formation of Plk1 and INCENP required for metaphase-anaphase transition." in: Nature cell biology, Vol. 8, Issue 2, pp. 180-7, 2006 (PubMed).

Hosts (40), (16)
Reactivities (43), (7), (5), (5), (4), (4)
Applications (50), (49), (25), (7), (2), (2), (2), (1), (1), (1)
Conjugates (7), (7)
Epitopes (2), (2)
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