GANP antibody

Catalog No. ABIN265431

The Rabbit Polyclonal anti-GANP antibody (ABIN265431) specifically detects GANP in WB and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for GANP antibody (ABIN265431)

Target: GANP (MCM3AP) (Minichromosome Maintenance Complex Component 3 Associated Protein (MCM3AP))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GANP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GANP Antibody

Specificity: This antibody detects endogenous levels of GANP/MCM3AP protein. (region surrounding Glu1872)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GANP

Target: GANP (MCM3AP) (Minichromosome Maintenance Complex Component 3 Associated Protein (MCM3AP))

Alternative Name: MCM3AP

Background: Minichromosome maintenance protein 3 (MCM3) is a protein essential for the initiation of DNA replication. MCM3-associated protein (MCM3AP), also known as GANP or MAP80, is a 1980 amino acid protein which binds to MCM3. MCM3AP is an acetyltransferase that acetylates MCM3, thereby playing an indirect role in DNA replication. MCM3AP is also believed to be involved in the nuclear localization of MCM3. Localized to the nucleus and the cytoplasm, GANP (MCM3AP) is thought to have phosphorylation-dependent DNA-primase activity that is up-regulated in germinal center regions. MCM3AP contains a nuclear localization signal, a coiled-coil structure and four LXXLL motifs.Synonyms: GANP, KIAA0572, MAP80, MCM3 minichromosome maintenance deficient 3 associated protein

Molecular Weight: approx. 218 kDa

Gene ID: 8888

NCBI Accession: NP_003897

UniProt: O60318

Pathways: Protein targeting to Nucleus