LIM Domain Kinase 1 antibody

Catalog No. ABIN265508

The Rabbit Polyclonal anti-LIM Domain Kinase 1 antibody is suitable to detect LIM Domain Kinase 1 in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for LIM Domain Kinase 1 antibody (ABIN265508)

Target: LIM Domain Kinase 1 (LIMK1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIM Domain Kinase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LIM Domain Kinase 1 Antibody

Specificity: This antibody detects endogenous levels of LIMK1/2 protein. (region surrounding Asp402)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Also reacts to: LIMK2

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for LIM Domain Kinase 1

Target: LIM Domain Kinase 1 (LIMK1)

Alternative Name: LIMK1

Background: Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, including LIMK-1 and LIMK-2, has been identified. LIMK-1 has been shown to regulate the stabilization of F-Actin structures and cofilin activity, indicating that LIMK-1 plays a role in a signaling pathway involved in the regulation of cell motility and morphogenesis. LIMK-1 inhibits neuronal differentiation of PC12 cells, and is thought to act by interfering with events downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest that these proteins may have different functions during development. A truncated form of LIMK-2 has been identified in adult testis that is thought to arise from an alternative initiation exon.Synonyms: EC=2.7.11.1, LIM domain kinase 1, LIMK, LIMK-1

Molecular Weight: approx. 72 kDa

Gene ID: 3984

NCBI Accession: NP_002305

UniProt: P53667

Pathways: Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events