PAR1 antibody

Catalog No. ABIN272129

The Rabbit Polyclonal anti-PAR1 antibody is suitable to detect PAR1 in samples from Human. It has been validated for WB.

Quick Overview for PAR1 antibody (ABIN272129)

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAR1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PAR1 Antibody

Specificity: This antibody detects endogenous levels of PAR1 protein. (region surrounding Gly17)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/10000approx. 1/20000. Western blot: 1/500approx. 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in alqiuots) at -20 °C for longer.

Target Details for PAR1

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Alternative Name: Thrombin Receptor / F2R

Background: Thrombin receptor (also designated protease-activated receptor-1 or PAR-1), PAR-2 and PAR-3 compose a distinct class of G protein-coupled receptors activated by proteolysis. Cleavage of these receptors by proteases occurs within the amino-terminal extracellular domain. Thrombin, a serine protease involved in platelet aggregation and blood coagulation, activates the thrombin receptor, resulting in elevated intracellular calcium levels in platelets. Thrombin also cleaves PAR-3 in vitro, suggesting that PAR-3 may be involved in thrombosis or mitogenesis. Thrombin receptor and PAR-4 appear to account for most thrombin signaling in platelets. Activation of PAR-2 in vitro is induced by trypsin, suggesting that PAR-2 is not an alternative thrombin receptor. Cytokines including TNF-alpha and IL-1beta increase PAR-2 expression, indicating PAR-2 involvement in the acute inflammatory response.Synonyms: CF2R, Coagulation factor II receptor, PAR-1, PAR1, Proteinase-activated receptor 1

Molecular Weight: approx. 48 kDa

Gene ID: 2149

NCBI Accession: NP_001983

UniProt: P25116

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus