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Activating Transcription Factor 1 (AFT1) antibody

Antigen

Activating Transcription Factor 1 (AFT1)
Synonyms TREB36, EWS-ATF1, FUS/ATF-1, Atf-1, zgc:64099, wu:fc92c07, treb36, MGC89098, ews-atf1, fus/atf-1, ATF1, MGC143110
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Application
Alternatives Enzyme Immunoassay (EIA), Western Blotting (WB)
Catalog no. ABIN272179
Quantity 100 µg  (1,0 mg/ml)  (Variants)
Price 340.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Characteristics Synonyms: ATF-1, Activating transcription factor 1, Protein TREB36, Cyclic AMP-dependent transcriptionfactor ATF-1, cAMP-dependent transcription factor ATF-1
Alternative name ATF1
Swiss-Prot P18846
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Description Eukaryotic gene transcription is regulated by sequence-specific transcription factors thatbind modular cis acting promoter and enhancer elements. The ATF/ CREB transcriptionfactor family binds the palindromic cAMP response element (CRE) octanucleotideTGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1,ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, butshare a C-terminal leucine zipper for dimerization and DNA binding. ATF-1 is shown to playa key role in the induction of NOX1, this protein binds the cAMP response element (CRE)and mediates PKA-induced stimulation of CRE-reporter genes. ATF-2 forms homodimersand heterodimers with c-Jun to initiate CREdependent transcription. Phosphorylation ofATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptionalactivation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong thehalf-life of ATF-2. ATF-2 functions as a histone acetyltransferase (HAT) and acetylatesHistones H2B and H4 specifically in vitro.
Specificity ATF1 (T209) antibody detects endogenous levels of ATF1 protein. Species: Human, Mouse, Rat. Others not tested.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes ELISA: 1: 5000~1: 10000. WB: 1: 500~1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 1,0 mg/ml
Purification Aff - Purified
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Storage Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "Activating Transcription Factor 1 (AFT1)", type "Antibodies"
Hosts Rabbit (28), Mouse (6), Goat (3), Sheep (1)
Reactivities Human (35), Mouse (Murine) (23), Rat (Rattus) (23), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1), Guinea Pig (1), Horse (Equine) (1), Pig (Porcine) (1), Rabbit (1), Yeast (Saccharomyces Cerevisiae) (1)
Applications Immunofluorescence (IF) (20), Western Blotting (WB) (20), Flow Cytometry (FACS) (18), ELISA (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunoprecipitation (IP) (4), Enzyme Immunoassay (EIA) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)