MED1 antibody

Catalog No. ABIN272236

This Rabbit Polyclonal antibody specifically detects MED1 in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for MED1 antibody (ABIN272236)

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MED1 Antibody

Specificity: This antibody detects endogenous levels of TRAP220 protein. (region surrounding Pro697)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MED1

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Alternative Name: MED1

Background: In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signaling between transcriptional activators and initiation factors. These complexes include the thyroid hormone receptor-associated protein (TRAP) complex, which interacts with thyroid receptors (TR), vitamin D receptors and other steroid receptors to facilitate hormone induced transcriptional activation. The TRAP complex consists of numerous proteins ranging in size from 20 to 240 kDa, including TRAP95, TRAP100, TRAP150, TRAP220 and TRAP230, that are characterized by the presence of a nuclear receptor recognition motif which mediates the liganddependent binding of TRAP proteins to the nuclear receptors. TRAP220 and TRAP100 are widely expressed and most abundantly detected in skeletal muscle, heart and placenta. TRAP95, TRAP150 and TRAP230 facilitate TR induced transcription by associating with an additional transcriptional coactivating complex SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes.Synonyms: ARC205, Activator-recruited cofactor 205 kDa component, CRSP1, CRSP200, DRIP205, DRIP230, Mediator complex subunit 1, PBP, PPARBP, PPARGBP, Peroxisome proliferator-activated receptor-binding protein, RB18A, TRAP220, TRIP2, Thyroid hormone receptor-associated protein complex 220 kDa component, Thyroid receptor-interacting protein 2, Vitamin D receptor-interacting protein complex component DRIP205, p53 regulatory protein RB18A

Molecular Weight: approx. 170 kDa

Gene ID: 5469

NCBI Accession: NP_004765

UniProt: Q15648

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha