DGKD antibody

Catalog No. ABIN272240

The Rabbit Polyclonal anti-DGKD antibody is suitable to detect DGKD in samples from Human. It has been validated for WB, IF and IHC (p).

Quick Overview for DGKD antibody (ABIN272240)

Target: DGKD (Diacylglycerol Kinase, delta 130kDa (DGKD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGKD antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DGKD Antibody

Specificity: This antibody detects endogenous levels of DGK-δ protein. (region surrounding Ser66)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DGKD

Target: DGKD (Diacylglycerol Kinase, delta 130kDa (DGKD))

Alternative Name: DAG Kinase delta

Background: Diacylglycerol kinases (DGKs) phosphorylate diacylglycerol (DAG) to produce phosphatidic acid. DAG and phosphatidic acid are lipids that act as second messengers in signaling cascades. DGK-α influences cell activation and secretion of lethal exosomes, which in turn control cell death. DGK-β is abundant in restricted brain regions such as the caudate putamen and olfactory tubercle. DGK-γ encodes full-length and truncated transcripts that are present in a range of human tissues, with greatest expression observed in retina. DGK-δ is most abundant in skeletal muscle. DGK-ε shows specificity for arachidonylcontaining diacylglycerol and is expressed predominantly in testis. DGK-θ is most abundant in the cerebellum and hippocampus. DGK-ι is present in brain and retina as a predominant transcript of more than 12 kb, including a long 3-prime untranslated region, with additional low abundance transcripts of 9.5 and 7.5 kb. DGK-η is closely related to DGK-δ. DGK-ζ is most abundant in brain and muscle. DGKs have structural motifs that play regulatory roles, and these motifs form the basis for dividing the DGKs into five subtypes.Synonyms: DGK-delta, DGKD, Diacylglycerol kinase delta, Diglyceride kinase delta, KIAA0145

Molecular Weight: approx. 135 kDa

Gene ID: 8527

NCBI Accession: NP_003639

UniProt: Q16760

Pathways: EGFR Signaling Pathway