ELF5 antibody

Catalog No. ABIN272261

The Rabbit Polyclonal anti-ELF5 antibody is suitable to detect ELF5 in samples from Human and Mouse. It has been validated for WB and IF.

Quick Overview for ELF5 antibody (ABIN272261)

Target: ELF5 (E74-Like Factor 5 (Ets Domain Transcription Factor) (ELF5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELF5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-ELF5 Antibody

Specificity: This antibody detects endogenous levels of Elf-5 protein. (region surrounding Trp215)

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/20000-1/40000. Western Blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ELF5

Target: ELF5 (E74-Like Factor 5 (Ets Domain Transcription Factor) (ELF5))

Alternative Name: ELF5 / ESE2

Background: Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1-3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA-binding activity of all members of the Ets gene family. Elf-5 is a member of the Ets family that may be involved in lung, mammary, prostate and kidney function, and may also play a role in tumorigenesis.Synonyms: E74-like factor 5, ETS-related transcription factor Elf-5, Elf-5, Epithelium-restricted ESE-1-related Ets factor, Epithelium-specific Ets transcription factor 2

Molecular Weight: approx. 31 kDa

Gene ID: 2001

NCBI Accession: NP_001413

UniProt: Q9UKW6