tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, epsilon Polypeptide (YWHAE) antibody

Antigen: tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, epsilon Polypeptide (YWHAE)

Synonyms: MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465, AU019196, 14-3-3e, 14-3-3, 14-3-3-e, CT24092, D14-3-3e, D14-3-3epsilon, EK3-5, PAR5, Par-5, SR3-9, Su(Raf)3B, anon-WO0172774.141, anon-WO02059370.52, l(3)j2B10, DmelCG31196, CG31196, LOC443300, YWHAE, mds, mdcr, kcip-1, MGC89915, 14-3-3epsilon, LOC581376, Ywhae, ACYPI002517, ywhae, zgc:55377, zgc:77062, wu:fb48f06, wu:fi22e10, MGC53170

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN272265

Additional Information

Characteristics: Synonyms: 14-3-3E, YWHAE

Alternative name: 14-3-3 protein epsilon

Swiss-Prot: P62258

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notablyapoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise thisfamily of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins formdimers that present two binding sites for ligand proteins, thereby bringing together twoproteins that may not otherwise associate. These ligands largely share a 14-3-3 consensusbinding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function inbroad regulation of these ligand proteins, by cytoplasmic sequestration, occupation ofinteraction domains and import/export sequences, prevention of degradation,activation/repression of enzymatic activity and facilitation of protein modification, andthus loss of expression contributes to a vast array of pathogenic cellular activities.

Specificity: 14-3-3 ε (D238) antibody detects endogenous levels of 14-3-3 ε protein. Species: Human, Mouse, Rat. Others not tested.

Synonyms: MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465, AU019196, 14-3-3e, 14-3-3, 14-3-3-e, CT24092, D14-3-3e, D14-3-3epsilon, EK3-5, PAR5, Par-5, SR3-9, Su(Raf)3B, anon-WO0172774.141, anon-WO02059370.52, l(3)j2B10, DmelCG31196, CG31196, LOC443300, YWHAE, mds, mdcr, kcip-1, MGC89915, 14-3-3epsilon, LOC581376, Ywhae, ACYPI002517, ywhae, zgc:55377, zgc:77062, wu:fb48f06, wu:fi22e10, MGC53170

Application Details

Application Notes: ELISA: 1: 20000~1: 40000. WB: 1: 500~1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1,0 mg/ml

Purification: Aff - Purified

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Storage: Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Research Area: Transcription Factors

Restrictions: For Research Use only