EGFR antibody

Catalog No. ABIN285476

The Sheep Polyclonal anti-EGFR antibody has been validated for WB, IHC (p), IP and IC. It is suitable to detect EGFR in samples from Human. There are 16+ publications available.

Quick Overview for EGFR antibody (ABIN285476)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunochromatography (IC)

Product Details anti-EGFR Antibody

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Turkey

Purification: purified

Immunogen: EGFR antibody was raised in sheep using a synthetic 20 amino acid peptide of human EGFr representing a portion of the internal domain proximal to a phosphorylation region as the immunogen.

Application Details

Application Notes: WB: 10-50 µg/mL, IC: 25-100 µg/mL, IHC-P: 5 µg/mL, IP: 10-12 µg/mL, WB: 10-50 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: Protein G purified and supplied in PBS, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

References for anti-EGFR antibody (ABIN285476)

Saveri, De Luca, Nisi, Pisciotta, Romano, Piscosquito, Reilly, Polke, Cavallaro, Fabrizi, Fossa, Cichero, Lombardi, Lauria, Magri, Taroni, Pareyson, Bucci: "Charcot-Marie-Tooth Type 2B: A New Phenotype Associated with a Novel RAB7A Mutation and Inhibited EGFR Degradation." in: Cells, Vol. 9, Issue 4, (2020) (PubMed).

Larios, Mercier, Roux, Gruenberg: "ALIX- and ESCRT-III-dependent sorting of tetraspanins to exosomes." in: The Journal of cell biology, Vol. 219, Issue 3, (2020) (PubMed).

Ripoll, Heiligenstein, Hurbain, Domingues, Figon, Petersen, Dennis, Houdusse, Marks, Raposo, Delevoye: "Myosin VI and branched actin filaments mediate membrane constriction and fission of melanosomal tubule carriers." in: The Journal of cell biology, Vol. 217, Issue 8, pp. 2709-2726, (2019) (PubMed).

Matsui, Jiang, Nakano, Sakamaki, Yamamoto, Mizushima: "Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17." in: The Journal of cell biology, Vol. 217, Issue 8, pp. 2633-2645, (2019) (PubMed).

Keir, Chandramohan, Hemphill, Grandal, Melander, Pedersen, Horak, Kragh, Desjardins, Friedman, Bigner: "Sym004-induced EGFR elimination is associated with profound anti-tumor activity in EGFRvIII patient-derived glioblastoma models." in: Journal of neuro-oncology, Vol. 138, Issue 3, pp. 489-498, (2019) (PubMed).

Wenzel, Schultz, Schink, Pedersen, Nähse, Carlson, Brech, Stenmark, Raiborg: "Concerted ESCRT and clathrin recruitment waves define the timing and morphology of intraluminal vesicle formation." in: Nature communications, Vol. 9, Issue 1, pp. 2932, (2018) (PubMed).

Torgersen, Klokk, Kavaliauskiene, Klose, Simons, Skotland, Sandvig: "The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport." in: Oncotarget, Vol. 7, Issue 52, pp. 86871-86888, (2018) (PubMed).

Filipek, de Araujo, Vogel, De Smet, Eberharter, Rebsamen, Rudashevskaya, Kremser, Yordanov, Tschaikner, Fürnrohr, Lechner, Dunzendorfer-Matt, Scheffzek, Bennett, Superti-Furga, Lindner, Stasyk, Huber: "LAMTOR/Ragulator is a negative regulator of Arl8b- and BORC-dependent late endosomal positioning." in: The Journal of cell biology, Vol. 216, Issue 12, pp. 4199-4215, (2017) (PubMed).

Fujita, Huang, Lin, Groulx, Jean, Nguyen, Kuchitsu, Koyama-Honda, Mizushima, Fukuda, Kiger: "Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy." in: eLife, Vol. 6, (2017) (PubMed).

Lo Cicero, Delevoye, Gilles-Marsens, Loew, Dingli, Guéré, André, Vié, van Niel, Raposo: "Exosomes released by keratinocytes modulate melanocyte pigmentation." in: Nature communications, Vol. 6, pp. 7506, (2016) (PubMed).

Matsui, Noguchi, Fukuda: "Dennd3 functions as a guanine nucleotide exchange factor for small GTPase Rab12 in mouse embryonic fibroblasts." in: The Journal of biological chemistry, Vol. 289, Issue 20, pp. 13986-95, (2014) (PubMed).

Rønning, Pedersen, Madshus, Stang: "CIN85 regulates ubiquitination and degradative endosomal sorting of the EGF receptor." in: Experimental cell research, Vol. 317, Issue 13, pp. 1804-16, (2011) (PubMed).

Larsen, Stockhausen, Poulsen: "Cell adhesion and EGFR activation regulate EphA2 expression in cancer." in: Cellular signalling, Vol. 22, Issue 4, pp. 636-44, (2010) (PubMed).

Grøvdal, Johannessen, Rødland, Madshus, Stang: "Dysregulation of Ack1 inhibits down-regulation of the EGF receptor." in: Experimental cell research, Vol. 314, Issue 6, pp. 1292-300, (2008) (PubMed).

Bertelsen, Breen, Sandvig, Stang, Madshus: "The Cbl-interacting protein TULA inhibits dynamin-dependent endocytosis." in: Experimental cell research, Vol. 313, Issue 8, pp. 1696-709, (2007) (PubMed).

Oksvold, Huitfeldt, Langdon: "Identification of 14-3-3zeta as an EGF receptor interacting protein." in: FEBS letters, Vol. 569, Issue 1-3, pp. 207-10, (2004) (PubMed).

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR

Background: The epidermal growth factor receptor (EGFR) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGF alpha).

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins