Alkaline Phosphatase antibody (HRP)

Catalog No. ABIN288216

The HRP-conjugated Rabbit Polyclonal anti-Alkaline Phosphatase antibody (ABIN288216) specifically detects Alkaline Phosphatase in WB and ELISA. The antibody is reactive with E. coli samples.

Quick Overview for Alkaline Phosphatase antibody (HRP) (ABIN288216)

Target: Alkaline Phosphatase (ALP)

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Alkaline Phosphatase antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Product Details anti-Alkaline Phosphatase Antibody (HRP)

Cross-Reactivity (Details): No cross reactivity against mammalian forms of alkaline Phosphatase.

Purification: Affinity chromatography purified

Immunogen: Alkaline phosphatase antibody (HRP) was raised in rabbit using alkaline phosphatase isolated from E.coli as the immunogen.

Application Details

Application Notes: ELISA: 1:4,000-1:20,000, WB: 1:500-1:2,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA, and 0.01 % gentamycin sulfate. DO NOT add Sodium azide. Immunoglobulin and protease free.

Handling Advice: Avoid repeated freeze/thaw cycles.
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Target Details for Alkaline Phosphatase

Target: Alkaline Phosphatase (ALP)

Alternative Name: Alkaline Phosphatase

Background: Alkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.