Dnmt2 antibody

Catalog No. ABIN290198

The Mouse Monoclonal anti-Dnmt2 antibody is suitable to detect Dnmt2 in samples from Human and Mouse. It has been validated for WB, IHC and IHC (p).

Quick Overview for Dnmt2 antibody (ABIN290198)

Target: Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Dnmt2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 102B1259-2

Product Details anti-Dnmt2 Antibody

Specificity: In mouse Dnmt2 transfected cell lysate, a 42 kD band is observed.

Purification: Protein G purified

Immunogen: Synthetic peptides corresponding to mouse Dnmt2.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Dnmt2

Target: Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))

Alternative Name: TRDMT1 / DNMT2

Background: Name/Gene ID: TRDMT1
Family: C5-methyltransferase

Synonyms: TRDMT1, DMNT2, DNA MTase homolog HsaIIP, DNMT2, M.HsaIIP, MHSAIIP, RNMT1, DNA methyltransferase-2, PUMET

Gene ID: 1787

UniProt: O14717