UBASH3A antibody (AA 417-430)

Catalog No. ABIN290205

The Goat Polyclonal anti-UBASH3A antibody (ABIN290205) specifically detects UBASH3A in ELISA, IHC and IHC (p). The antibody is reactive with Human, Horse, Monkey, Bat and Dog samples.

Quick Overview for UBASH3A antibody (AA 417-430) (ABIN290205)

Target: UBASH3A (Ubiquitin Associated and SH3 Domain Containing, A (UBASH3A))

Reactivity: Human, Horse, Monkey, Bat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This UBASH3A antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UBASH3A Antibody

Binding Specificity: AA 417-430

Specificity: Human UBASH3A. This antibody is expected to recognize both reported isoforms (NP_061834.1 and NP_001001895.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Dog, Bat, Panda, Horse (100%) Opossum (93%) Mouse, Bovine, Hamster (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide QQCSTPDGKYYRPD from an internal region of human UBASH3A (NP_061834.1, NP_001001895.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Dog, Bat, Panda, Horse (100%), Opossum (93%), Mouse, Bovine, Hamster (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:64000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for UBASH3A

Target: UBASH3A (Ubiquitin Associated and SH3 Domain Containing, A (UBASH3A))

Alternative Name: UBASH3A / CLIP4

Background: Name/Gene ID: UBASH3A

Synonyms: UBASH3A, STS2, TULA-1, STS-2, T-cell ubiquitin ligand 1, TULA, Cbl-interacting protein 4

Gene ID: 53347

NCBI Accession: NP_061834, NP_001001895

UniProt: P57075