GRP94 antibody (AA 590-640)

Catalog No. ABIN290247

The Rabbit Polyclonal anti-GRP94 antibody (ABIN290247) specifically detects GRP94 in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat, Cow, Chicken, Monkey, Horse, Xenopus laevis and Zebrafish (Danio rerio) samples.

Quick Overview for GRP94 antibody (AA 590-640) (ABIN290247)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivity: Human, Mouse, Rat, Cow, Chicken, Monkey, Horse, Xenopus laevis, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRP94 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GRP94 Antibody

Binding Specificity: AA 590-640

Specificity: A portion of amino acids 590-640 of human GRP94.

Purification: Protein G purified

Immunogen: A portion of amino acids 590-640of human GRP94.

Type of Immunogen: Protein fragment

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Alternative Name: HSP90B1 / GP96 / GRP94

Background: Name/Gene ID: HSP90B1

Synonyms: HSP90B1, ECGP, Endoplasmin, gp96 homolog, gp96, GRP-94, GRP94, Tumor rejection antigen 1, TRA1

Gene ID: 7184

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades