MAF antibody (AA 50-100)

Catalog No. ABIN290248

This Rabbit Polyclonal antibody specifically detects MAF in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog and Monkey.

Quick Overview for MAF antibody (AA 50-100) (ABIN290248)

Target: MAF (V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (Avian) (MAF))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MAF Antibody

Binding Specificity: AA 50-100

Specificity: A portion of amino acids 50-100 of human c-maf.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Stickleback, Pufferfish, Zebrafish (100%) Xenopus (94%) Opossum (88%) Chicken (81%).

Purification: Immunoaffinity purified

Immunogen: A synthetic peptide corresponding to amino acids 75-90 (APSPGSGSEQKAHLED) of human c-maf was used as the immunogen, GenBank no. NP_001026974.1|. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Stickleback, Pufferfish, Zebrafish (100%), Xenopus (94%), Opossum (88%), Chicken (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for MAF

Target: MAF (V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (Avian) (MAF))

Alternative Name: c-Maf

Background: Name/Gene ID: MAF

Synonyms: MAF, C-MAF, CCA4, C-maf proto-oncogene, Proto-oncogene c-Maf, T lymphocyte c-maf long form, Transcription factor Maf

Gene ID: 4094

NCBI Accession: NP_001026974

UniProt: O75444