ULK1 antibody (AA 320-370)

Catalog No. ABIN290381

The Rabbit Polyclonal anti-ULK1 antibody is suitable to detect ULK1 in samples from Human and Monkey. It has been validated for WB, IHC and IHC (p).

Quick Overview for ULK1 antibody (AA 320-370) (ABIN290381)

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ULK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ULK1 Antibody

Binding Specificity: AA 320-370

Specificity: A portion of amino acid 320-370 of human ULK1.

Purification: Protein G purified

Immunogen: A portion of amino acid320-370of human ULK1.

Type of Immunogen: Protein fragment

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for ULK1

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Alternative Name: ULK1

Background: Name/Gene ID: ULK1
Subfamily: ULK
Family: Protein Kinase

Synonyms: ULK1, ATG1A, ATG1, HATG1, KIAA0722, Unc-51-like kinase 1, Unc51.1, UNC51

Gene ID: 8408

UniProt: O75385

Pathways: Regulation of Cell Size, Autophagy