AGR2 antibody (AA 55-72)

Catalog No. ABIN291006

The Rabbit Polyclonal anti-AGR2 antibody (ABIN291006) specifically detects AGR2 in IHC, ELISA and IHC (p). The antibody is reactive with Human, Cow, Dog, Horse, Rabbit, Monkey and Pig samples.

Quick Overview for AGR2 antibody (AA 55-72) (ABIN291006)

Target: AGR2 (Anterior Gradient Homolog 2 (Xenopus Laevis) (AGR2))

Reactivity: Human, Cow, Dog, Horse, Rabbit, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGR2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AGR2 Antibody

Binding Specificity: AA 55-72

Specificity: Researchers are encouraged to use the sequence and accession number (NP_006399.1) that were used to design the peptide immunogen in order to assess potential cross-reactivity to their species of interest. Does not recognize AGR3 (Verma et al, 2012).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda, Dog, Bovine, Horse, Rabbit, Pig (100%) Rat, Hamster, Elephant, Bat, Opossum, Platypus (94%) Mouse (89%) Chicken (83%).

Purification: Protein G purified

Immunogen: This antibody was developed against synthetic peptides corresponding to amino acids 55-72 (TQTYEEALYKSKTSNKPL) of human AGR2. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda, Dog, Bovine, Horse, Rabbit, Pig (100%), Rat, Hamster, Elephant, Bat, Opossum, Platypus (94%), Mouse (89%), Chicken (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:100 - 1:1000), IHC, IHC-P (20 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS containing 0.05 % BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.

Target Details for AGR2

Target: AGR2 (Anterior Gradient Homolog 2 (Xenopus Laevis) (AGR2))

Alternative Name: AGR2

Background: Name/Gene ID: AGR2

Synonyms: AGR2, AG2, AG-2, HPC8, HAG-2, Secreted cement gland homolog, Anterior gradient homolog 2, GOB-4, PDIA17, XAG-2

Gene ID: 10551

UniProt: O95994