CCR10 antibody (AA 350-362)

Catalog No. ABIN291274

This Rabbit Polyclonal antibody specifically detects CCR10 in WB, IHC, FACS and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CCR10 antibody (AA 350-362) (ABIN291274)

Target: CCR10 (Chemokine (C-C Motif) Receptor 10 (CCR10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCR10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CCR10 Antibody

Binding Specificity: AA 350-362

Specificity: In A375, a ~50 kD band is observed. Is made against an epitope in the cytoplasmic domain of the receptor (Moed et al, 2004). For flow cytometry (intracellular) Moed et al fixed cells with 2 % formaldehyde and permeabilized with 0.1 % saponin.

Purification: Protein G purified

Immunogen: A peptide corresponding to amino acids 350-362 of human CCR10. In this region, mouse and human amino acid sequences are 100% identical.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: Flo, IHC, IHC-P (5 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CCR10

Target: CCR10 (Chemokine (C-C Motif) Receptor 10 (CCR10))

Alternative Name: CCR10 / GPR2

Background: Name/Gene ID: CCR10
Subfamily: Chemokine
Family: GPCR

Synonyms: CCR10, C-C CKR-10, CC-CKR-10, CCR-10, C-C chemokine receptor type 10, CC chemokine receptor 10, G-protein coupled receptor 2, GPR2, G protein-coupled receptor 2

Gene ID: 2826

UniProt: P46092