HDAC3 antibody (AA 2-17)

Catalog No. ABIN291844

This Rabbit Polyclonal antibody specifically detects HDAC3 in WB, IHC, IHC (p), IP and ChIP. It exhibits reactivity toward Human.

Quick Overview for HDAC3 antibody (AA 2-17) (ABIN291844)

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Product Details anti-HDAC3 Antibody

Binding Specificity: AA 2-17

Specificity: In HeLa, a 50 kD band is observed.

Purification: Protein G purified

Immunogen: Synthetic peptide from human HDAC3.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ChrIP, IHC, IHC-P (10 μg/mL), IP, WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for HDAC3

Target: HDAC3 (Histone Deacetylase 3 (HDAC3))

Alternative Name: HDAC3

Background: Name/Gene ID: HDAC3
Family: Histone Deacetylase

Synonyms: HDAC3, RPD3, SMAP45, HD3, Histone deacetylase 3, RPD3-2

Gene ID: 8841

UniProt: O15379

Pathways: Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development