ING3 antibody (AA 294-304)

Catalog No. ABIN291938

The Goat Polyclonal anti-ING3 antibody is suitable to detect ING3 in samples from Human. It has been validated for WB, ELISA, IHC, IF and IHC (p).

Quick Overview for ING3 antibody (AA 294-304) (ABIN291938)

Target: ING3 (Inhibitor of Growth Family, Member 3 (ING3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ING3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ING3 Antibody

Binding Specificity: AA 294-304

Specificity: Aa 294-304 of Human ING3 protein (Inhibitor of growth family, member 3). This sequence only shows homology to isoform 1 for ING3.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human ING3.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:40000), IF, IHC, IHC-P (2.5 μg/mL), WB (1:200 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for ING3

Target: ING3 (Inhibitor of Growth Family, Member 3 (ING3))

Alternative Name: ING3

Background: Name/Gene ID: ING3

Synonyms: ING3, p47ING3, Inhibitor of growth protein 3, MEAF4, Eaf4, p47

Gene ID: 54556

UniProt: Q9NXR8