Caveolin-1 antibody (AA 73-84)

Catalog No. ABIN294089

The Goat Polyclonal anti-Caveolin-1 antibody is suitable to detect Caveolin-1 in samples from Human and Monkey. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for Caveolin-1 antibody (AA 73-84) (ABIN294089)

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Caveolin-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Caveolin-1 Antibody

Binding Specificity: AA 73-84

Specificity: Human CAV1 / Caveolin. No cross-reactivity expected to Caveolin 2 and Caveolin 3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Marmoset (100%) Orangutan, Guinea pig (92%) Mouse, Rat, Dog, Bat, Hamster, Rabbit, Horse (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DELSEKQVYDAH from an internal region of human CAV1 / Caveolin (NP_001744.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Marmoset (100%), Orangutan, Guinea pig (92%), Mouse, Rat, Dog, Bat, Hamster, Rabbit, Horse (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Alternative Name: CAV1 / Caveolin 1

Background: Name/Gene ID: CAV1

Synonyms: CAV1, Caveolin-1, CGL3, CAV, MSTP085, VIP21, BSCL3

Gene ID: 857

NCBI Accession: NP_001744

UniProt: Q03135

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals