ERG antibody (AA 469-481)

Catalog No. ABIN295774

This Goat Polyclonal antibody specifically detects ERG in IHC, ELISA and IHC (p). It exhibits reactivity toward Human and Monkey.

Quick Overview for ERG antibody (AA 469-481) (ABIN295774)

Target: ERG (ERG, ETS transcription factor (ERG))

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This ERG antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ERG Antibody

Binding Specificity: AA 469-481

Specificity: Human ERG. This antibody is expected to recognise both reported isoforms (NP_891548.1, NP_004440.1)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Horse, Opossum, Platypus, Lizard, Xenopus (92%) Rabbit, Turkey, Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-PNTRLPTSHMPSH from the C-terminus of human ERG (NP_891548.1, NP_004440.1, NP_001129626.1, NP_001129627.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Horse, Opossum, Platypus, Lizard, Xenopus (92%), Rabbit, Turkey, Chicken (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), Peptide-ELISA (1:16000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ERG

Target: ERG (ERG, ETS transcription factor (ERG))

Alternative Name: ERG

Background: Name/Gene ID: ERG

Synonyms: ERG, ERG-3, Ets-related, TMPRSS2/ERG fusion, Transcriptional regulator ERG, p55, Transforming protein ERG

Gene ID: 2078

NCBI Accession: NP_891548, NP_004440, NP_001129626, NP_001129627

UniProt: P11308