Osteoprotegerin antibody (AA 20-37)

Catalog No. ABIN296455

This Mouse Monoclonal antibody specifically detects Osteoprotegerin in WB, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Osteoprotegerin antibody (AA 20-37) (ABIN296455)

Target: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Osteoprotegerin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 98A1071

Product Details anti-Osteoprotegerin Antibody

Binding Specificity: AA 20-37

Specificity: The Osteoprotegerin antibody, clone 98A1071, detects all forms of Osteoprotegerin including monomeric, dimeric and Osteoprotegerin bound to RANKL (Vandooren, 2008) as well as Osteoprotegerin bound to TRAIL (Sandra, 2006).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon (100%) Monkey (94%) Marmoset, Panda, Dog, Horse, Pig, Opossum (89%) Mouse, Rat, Elephant, Bovine (83%).

Purification: Protein G purified

Immunogen: A peptide corresponding to the amino acids 20-37 TQETFPPKYLHYDEETSH of human OPG. The specificity was tested on recombinant OPG by western blot analysis. Percent identity by BLAST analysis: Human, Gibbon (100%), Monkey (94%), Marmoset, Panda, Dog, Horse, Pig, Opossum (89%), Mouse, Rat, Elephant, Bovine (83%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide. Sourced in TCS.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Osteoprotegerin

Target: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Alternative Name: Osteoprotegerin / OPG

Target Type: Chemical

Background: Name/Gene ID: TNFRSF11B
Family: TNF Receptor

Synonyms: TNFRSF11B, OCIF, OPG, Osteoprotegerin, TR1

Gene ID: 4982

UniProt: O00300