ABCC4 antibody (AA 1312-1325)

Catalog No. ABIN296628

The Goat Polyclonal anti-ABCC4 antibody (ABIN296628) specifically detects ABCC4 in IHC, ELISA, IHC (p), ICC and IF. The antibody is reactive with Human and Monkey samples.

Quick Overview for ABCC4 antibody (AA 1312-1325) (ABIN296628)

Target: ABCC4 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 4 (ABCC4))

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This ABCC4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-ABCC4 Antibody

Binding Specificity: AA 1312-1325

Specificity: Human ABCC4 / MRP4. This antibody is expected to recognize isoform 1 (NP_005836.2) only.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Mouse, Rat, Hamster, Elephant, Horse, Rabbit (93%) Marmoset, Dog (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-NGQPSTLTIFETAL from the C-terminus of human ABCC4 / MRP4 (NP_005836.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Mouse, Rat, Hamster, Elephant, Horse, Rabbit (93%), Marmoset, Dog (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ICC, IF, IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:64000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ABCC4

Target: ABCC4 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 4 (ABCC4))

Alternative Name: ABCC4 / MRP4

Background: Name/Gene ID: ABCC4
Subfamily: ATP-binding cassette - ABCC/MRP
Family: Transporter

Synonyms: ABCC4, EST170205, MOAT-B, Multidrug resistance protein 4, MOATB, MRP4

Gene ID: 10257

NCBI Accession: NP_005836

UniProt: O15439